王宏宇实验室

研究方向：

       糖脂代谢调控以及代谢综合症。利用基因工程大小鼠模型结合生物化学、生理学、蛋白组学以及代谢组学等手段阐明维持机体糖脂代谢稳态的分子机制，探讨代谢疾病发生、发展的机理，为代谢综合症的临床诊疗提供理论依据。主要研究方向：1）非酒精性脂肪肝/非酒精性脂肪肝炎的发病机制；2）代谢疾病相关动物模型的研发及治疗评价。

1.    Chen Q, Rong P, Xu DJ, Chen L, Xie B, Sheng Y, Li P, Wang HY* and Chen S*. (2017) Muscle-specific Rab8a deletion impairs lipid uptake and storage in skeletal muscle and causes hyperlipidemia and hepatosteatosis in mice. Diabetes. 66(9):2387-2399. (*corresponding author)

2.    Chen L, Chen Q, Rong P, Wang HY* and Chen S*. (2017) The energy sensing LKB1-AMPKα1 pathway regulates IGF1 secretion and consequent activation of the IGF1R-PKB pathway in primary hepatocytes. FEBS J. 2017 Jul; 284(13):2096-2109 (*corresponding author)

3.    Chen Q, Xie B, Zhu S, Rong P, Sheng Y, Ducommun S, Chen L, Quan C, Li M, Sakamoto K, MacKintosh C, Chen S* and Wang HY* (2017) A TBC1D1Ser231Ala knockin mutation partially impairs AICAR- but not exercise-induced muscle glucose uptake in mice. Diabetologia 60(2): 336-345 (*corresponding author)

4.    Xie B, Chen Q, Chen L, Sheng Y, Wang HY*, Chen S*. (2016) The Inactivation of RabGAP Function of AS160 Promotes Lysosomal Degradation of GLUT4 and Causes Postprandial Hyperglycemia and Hyperinsulinemia. Diabetes. Nov 65(11):3327-3340. (＊corresponding author)

5.    Chen L, Chen Q, Xie B, Quan C, Sheng Y, Zhu S, Rong P, Zhou S, Sakamoto K, MacKintosh C, Wang HY*, Chen S*. (2016) Disruption of the AMPK-TBC1D1 nexus increases lipogenic gene expression and causes obesity in mice via promoting IGF1 secretion. Proc Natl Acad Sci U S A. Jun 28; 113(26): 7219-24. (＊corresponding author)

6.    Wang HY＊, Quan C, Hu B, Xie, BX, Du Y, Chen L, Yang W, Yang L, Chen Q, Shen B, Hu B, Zheng ZH, Zhu HB, Huang XX, Xu GW, Chen S. (2016) A lipidomics study reveals hepatic lipid signatures associating with deficiency of the LDL receptor in a rat model. Biol Open. Jul 15; 5(7): 979-86. (＊corresponding author)

7.    杨葳，周生来，董婉维，王惟，于洋，郭晓冲，张婀娜，王宏宇*，郑志红*。(2015) eNOS基因敲除大鼠模型的建立及表达初步研究。实验动物与比较医学，35（4）：271-276. (＊corresponding author)

8.    徐影琪，李晓晶，杨葳，周生来，于洋，王惟，张婀娜，赵文，张梅英，郭大勇，王宏宇*，郑志红*。(2015) 应用Talen法制备apoE 基因敲除大鼠模型。实验动物与比较医学。35（4）：277-282. （＊corresponding author)

9.    Li M, Quan C, Toth R, Campbell DG, MacKintosh C*, Wang HY*, Chen S*. (2015) Fasting and systemic insulin signaling regulate phosphorylation of brain proteins that modulate cell morphology and link to neurological disorders. J Biol C 2015 Dec 11; 290 (50): 30030-41.  (＊corresponding author)

10.  Quan, C, Xie BX, Wang HY*, Chen S*. (2015) PKB-Mediated Thr649 Phosphorylation of AS160/TBC1D4 Regulates the R-Wave Amplitude in the Heart. PLoS One. Apr 29;10(4):e0124491(＊corresponding author)

11.  Chen Q, Quan C, Xie B, Chen L, Zhou S, Toth R, Campbell DG, Lu S, Shirakawa R, Horiuchi H, Li C, Yang Z, MacKintosh C, Wang HY*, Chen S*. (2014) GARNL1, a major RalGAP α subunit in skeletal muscle, regulates insulin-stimulated RalA activation and GLUT4 trafficking via interaction with 14-3-3 proteins. Cell Signal. 26(8):1636-48. (＊corresponding author)

12.  Wang H-Y, Ducommun S, Quan C, Xie BX., Li M., Wasserman DH, Sakamoto  K, MacKintosh C. and Chen S. (2013) AS160 deficiency causes whole-body insulin resistance via composite effects in multiple tissues. Biochem J. 449 (2): 479-489

13.  Yamada Y, Kubohara Y, Kikuchi H, Oshima Y, Wang H-Y, Ross S, Williams JG. (2013) The Dictyostelium prestalk inducer DIF-1 directs phosphorylation of a bZIP transcription factor. Int J Dev Biol. 57 (5) :375-381

14.  Ducommun S, Wang H-Y, Sakamoto K, MacKintosh C and Chen S. (2012) Thr649Ala-AS160 knockin mutation does not impair contraction/AICAR-induced glucose transport in mouse muscle. Am J Physiol Endocrinol Metab. 302: E1036-E1043

15.  Senoo H, Wang H-Y, Araki T, Williams JG, Fukuzawa M. (2012) An orthologue of the Myelin-gene Regulatory Transcription Factor regulates Dictyostelium prestalk differentiation. Int J Dev Biol. 56 (5) : 325-332

16.  Pears, C. J., Couto, C. A.-M., Wang H.-Y., Borer C., Kiely R. and Lakin, N. D. (2012) The role of ADP-ribosylationin regulating DNA double-stand break repair. Cell cycle. 11 (1): 48-56

17.  Couto C A-M, Wang H-Y, Green J, Kiely R, Siddaway R, Borer C, Pears C J and Lakin N D. (2011) PARP regulates non-homologous end-joining through retention of Ku at double strand breaks. J Cell Biol. 194: 367-375

18.  Hsu, D.-W., Kiely R, Couto C A-M, Wang H-Y, Hudson JJR, Borer C, Pears CJ and Lakin ND. (2011) DNA double-strand break repair pathway choice in Dictyostelium. J Cell Sci. 124 (10): 1655-1663

19.  Wang, H-Y, Williams, JG. (2010) Synergy between two transcription factors directs gene expression in Dictyostelium tip-organiser cells. Int. J Dev. Biol. 54(8-9): 1301-7

20.  Wang H-Y and Williams JG. (2010) Identification of a target for CudA the transcription factor that directs formation of the Dictyostelium tip organiser. Int. J Dev. Biol. 54(1): 161-5

21.  Yamada Y*, Wang H-Y*, Fukuzawa, M., Barton GJ, Williams JG. (2008) A new family of transcription factors. Development. 135(18): 3093-101. (* Co-first author)