秦进中实验室

秦进中，2000年1月赴美国克利夫兰州立大学生物学系攻读博士学位。博士课题在克利夫兰医学中心Xiaoxia Li实验室完成，主要利用转基因和基因剔除小鼠模型研究白介素1／Toll 样受体（IL-1/TLR)介导的细胞内信号通路及其免疫调节功能。2005年7月-2008 年3月于美国麻省总医院肿瘤中心，哈佛干细胞及再生医学研究所，哈佛大学医学院从事博士后研究工作，并在2008年4月被提升为遗传助理（Assistant in Genetics)。这期间主要从事干细胞增殖分化分子机制的研究。2013年12月通过南京大学引进高层次人才计划回国，受聘于南京大学模式动物研究所。主要研究方向针对干细胞增殖与分化的的表观遗传调控机制，着重以L3mbtl2介导的非典型多梳蛋白（POLYCOMB)复合物为模型，运用包括小鼠遗传学，基因组学和疍白组学等多学科手段，探寻表观 遗传机制在基因调控、干细胞增殖与分化、胚胎发育和肿瘤发生中的作用，最终结合所获得知 识促进干细胞替代疗法的临床应用，并提供合理而独特的药物设计线索治疗肿瘤等疾病。

Selected Publications(其中带*为通讯作者）

1.    Huang Y, Su T, Wang C, Dong L, Liu S, Zhu Y, Hao K, Xia Y, Jiang Q, Qin J^*. Rbbp4 Suppresses Premature Differentiation of Embryonic Stem Cells. Stem Cell Reports. 2021 Mar 9;16(3):566-581.

2.    Qin J^*, Wang C, Zhu Y, Su T, Dong L, Huang Y, Hao K. Mga safeguards embryonic stem cells from acquiring extraembryonic endoderm fates. Sci Adv. 2021 Jan 20;7(4):eabe5689.

3.    Liu M, Zhu Y, Xing F, Liu S, Xia Y, Jiang Q, Qin J^*. The polycomb group protein PCGF6 mediates germline gene silencing by recruiting histone-modifying proteins to target gene promoters. J Biol Chem. 2020 Jul 10;295(28):9712-9724.

4.    Zhao W., Liu M., Ji H., Zhu Y., Wang C., Huang Y., Ma X., Xing G., Xia Y., Jiang Q., Qin J*. 2018. The polycomb group protein Yaf2 regulates the pluripotency of embryonic stem cells in a phosphorylation-dependent manner. J Biol Chem.293(33):12793-12804.

5.    Lethal (3) malignant brain tumor-like 2 (L3MBTL2) protein protects against kidney injury by inhibiting the DNA damage-p53-apoptosis pathway in renal tubular cells. Huang H, Xu C, Wang Y, Meng C, Liu W, Zhao Y, Huang XR, You W, Feng B, Zheng ZH, Huang Y, Lan HY, Qin J^*, Xia Y^*. Kidney Int. 2018 Apr;93(4):855-870.

6.    Huang Y, Zhao W, Wang C, Zhu Y, Liu M, Tong H, Xia Y, Jiang Q, Qin J^*. Combinatorial Control of Recruitment of a Variant PRC1.6 Complex in Embryonic Stem Cells. Cell Rep. 2018 Mar 13;22(11):3032-3043.

7.    Liu W, Chen B, Wang Y, Meng C, Huang H, Huang XR, Qin J, Mulay SR, Anders HJ, Qiu A, Yang B, Freeman GJ, Lu HJ, Lin HY, Zheng ZH, Lan HY, Huang Y, Xia Y. RGMb protects against acute kidney injury by inhibiting tubular cell necroptosis via an MLKL-dependent mechanism. Proc Natl Acad Sci U S A. 2018 Feb 13;115(7):E1475-E1484.

8.    Zhao W, Huang Y, Zhang J, Liu M, Ji H, Wang C, Cao N, Li C, Xia Y, Jiang Q, Qin J^*. Polycomb group RING finger proteins 3/5 activate transcription via an interaction with the pluripotency factor Tex10 in embryonic stem cells. J Biol Chem. 2017 Dec 29;292(52):21527-21537.

9.    Yan Y, Zhao W, Huang Y, Tong H, Xia Y, Jiang Q, Qin J^*. Loss of Polycomb Group Protein Pcgf1 Severely Compromises Proper Differentiation of Embryonic Stem Cells. Sci Rep. 2017 Apr 10;7:46276.

10.  Zhou S, Lu W, Chen L, Ge Q, Chen D, Xu Z, Shi D, Dai J, Li J, Ju H, Cao Y, Qin J, Chen S, Teng H, Jiang Q. AMPK deficiency in chondrocytes accelerated the progression of instability-induced and ageing-associated osteoarthritis in adult mice. Sci Rep. 2017 Feb 22;7:43245.

11.  Zhao W, Tong H, Huang Y, Yan Y, Teng H, Xia Y, Jiang Q, Qin J^*. Essential Role for Polycomb Group Protein Pcgf6 in Embryonic Stem Cell Maintenance and a Noncanonical Polycomb Repressive Complex 1 (PRC1) Integrity. J Biol Chem. 2017 Feb 17;292(7):2773-2784.

12.  Meng H, Cao Y, Qin J, Song X, Zhang Q, Shi Y, Cao L. DNA methylation, its mediators and genome integrity. Int J Biol Sci. 2015 Apr 8;11(5):604-17.

13.  Hou H, Zheng K, Wang G, Ikegawa S, Zheng M, Gao X, Qin J, Teng H, Jiang Q. Influence of intra-articular administration of trichostatin a on autologous osteochondral transplantation in a rabbit model. Biomed Res Int. 2015;2015:470934.

14.  Foudi A, Kramer DJ, Qin J, Ye D, Behlich AS, Mordecai S, Preffer FI, Amzallag A, Ramaswamy S, Hochedlinger K, Orkin SH, Hock H. Distinct, strict requirements for Gfi-1b in adult bone marrow red cell and platelet generation. J Exp Med. 2014 May 5;211(5):909-27.

15.  Qin J, Whyte WA, Anderssen E, Apostolou E, Chen HH, Akbarian S, Bronson RT, Hochedlinger K, Ramaswamy S, Young RA, Hock H. The polycomb group protein L3mbtl2 assembles an atypical PRC1-family complex that is essential in pluripotent stem cells and early development. Cell Stem Cell. 2012 Sep 7;11(3):319-32.

16.  Qin J, Van Buren D, Huang HS, Zhong L, Mostoslavsky R, Akbarian S, Hock H. Chromatin protein L3MBTL1 is dispensable for development and tumor suppression in mice. J Biol Chem. 2010 Sep 3;285(36):27767-75.

17.  Schindler JW, Van Buren D, Foudi A, Krejci O, Qin J, Orkin SH, Hock H. TEL-AML1 corrupts hematopoietic stem cells to persist in the bone marrow and initiate leukemia. Cell Stem Cell. 2009 Jul 2;5(1):43-53.

18.  Bulek K, Swaidani S, Qin J, Lu Y, Gulen MF, Herjan T, Min B, Kastelein RA, Aronica M, Kosz-Vnenchak M, Li X. The essential role of single Ig IL-1 receptor-related molecule/Toll IL-1R8 in regulation of Th2 immune response. J Immunol. 2009 Mar 1;182(5):2601-9.

19.  Xiao H^D, Gulen MF^D, Qin J^D, Yao J, Bulek K, Kish D, Altuntas CZ, Wald D, Ma C, Zhou H, Tuohy VK, Fairchild RL, de la Motte C, Cua D, Vallance BA, Li X. The Toll-interleukin-1 receptor member SIGIRR regulates colonic epithelial homeostasis, inflammation, and tumorigenesis. Immunity. 2007 Apr;26(4):461-75. (D co-first).

20.  Yao J, Kim TW, Qin J, Jiang Z, Qian Y, Xiao H, Lu Y, Qian W, Gulen MF, Sizemore N, DiDonato J, Sato S, Akira S, Su B, Li X. Interleukin-1 (IL-1)-induced TAK1-dependent Versus MEKK3-dependent NFkappaB activation pathways bifurcate at IL-1 receptor-associated kinase modification. J Biol Chem. 2007 Mar 2;282(9):6075-89.

21.  Qin J, Yao J, Cui G, Xiao H, Kim TW, Fraczek J, Wightman P, Sato S, Akira S, Puel A, Casanova JL, Su B, Li X. TLR8-mediated NF-kappaB and JNK activation are TAK1-independent and MEKK3-dependent. J Biol Chem. 2006 Jul 28;281(30):21013-21.

22.  Schmitz J, Owyang A, Oldham E, Song Y, Murphy E, McClanahan TK, Zurawski G, Moshrefi M, Qin J, Li X, Gorman DM, Bazan JF, Kastelein RA. IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines. Immunity. 2005 Nov;23(5):479-90.

23.  Qin J, Qian Y, Yao J, Grace C, Li X. SIGIRR inhibits interleukin-1 receptor- and toll-like receptor 4-mediated signaling through different mechanisms. J Biol Chem. 2005 Jul 1;280(26):25233-41.

24.  Li X, Qin J. Modulation of Toll-interleukin 1 receptor mediated signaling. J Mol Med (Berl). 2005 Apr;83(4):258-66.

25.  Li X, Qin J.  SIGIRR/TIR8, a negative regulator of Toll-IL-1R signaling. Curr Med Chem.-Anti-Inflammatory & Anti-Allergy Agents, 2005; 4(1): 21-27.

26.  Qian Y, Qin J, Cui G, Naramura M, Snow EC, Ware CF, Fairchild RL, Omori SA, Rickert RC, Scott M, Kotzin BL, Li X. Act1, a negative regulator in CD40- and BAFF-mediated B cell survival. Immunity. 2004 Oct;21(4):575-87.

27.  Qin J, Jiang Z, Qian Y, Casanova JL, Li X. IRAK4 kinase activity is redundant for interleukin-1 (IL-1) receptor-associated kinase phosphorylation and IL-1 responsiveness. J Biol Chem. 2004 Jun 18;279(25):26748-53.

28.  Sakamoto S, Qin J, Navarro A, Gamero A, Potla R, Yi T, Zhu W, Baker DP, Feldman G, Larner AC. Cells previously desensitized to type 1 interferons display different mechanisms of activation of stat-dependent gene expression from naïve cells. J Biol Chem. 2004 Jan 30;279(5):3245-53.

29.  Wald D, Qin J, Zhao Z, Qian Y, Naramura M, Tian L, Towne J, Sims JE, Stark GR, Li X. SIGIRR, a negative regulator of Toll-like receptor-interleukin 1 receptor signaling. Nat Immunol. 2003 Sep;4(9):920-7

30.  Jiang Z, Johnson HJ, Nie H, Qin J, Bird TA, Li X. Pellino 1 is required for interleukin-1 (IL-1)-mediated signaling through its interaction with the IL-1 receptor-associated kinase 4 (IRAK4)-IRAK-tumor necrosis factor receptor-associated factor 6 (TRAF6) complex. J Biol Chem. 2003 Mar 28;278(13):10952-6.