刘耕实验室

研究方向：

细胞应激以及细胞代谢对细胞稳态的调控 
       刘耕课题组通过建立体内和体外一系列疾病模型和独特的报告、操作体系， 深入研究细胞应激和细胞代谢对细胞功能和稳态的维持和调控作用。通过解析细胞行为和细胞应激及细胞代谢之间的紧密互作关系，将有助于深入理解在发育、稳态维持、衰老以及肿瘤发生中复杂细胞行为的调控机制。以此为基础，刘耕课题组还进行转化应用方面的探索，涉及肿瘤免疫治疗以及退行性疾病干预等方面。

主要研究论文：* corresponding author

1．Xie YY^#, Zhang YN^#, Sun AN^#, Peng YM, Hou WK, Xiang C, Zhang GX, Lai BB, Hou XS, Zheng FF, Wang F and Liu G*. The coupling of mitoproteolysis and oxidative phosphorylation enables tracking of an active mitochondrial state through MitoTimer fluorescence. Redox Biology 2022, 56,102447. 

2．Wang Y, Zhang G, Meng Q, Huang S, Guo P, Leng Q, Sun L, Liu G*, Huang X* and Liu J*. Precise tumor immune rewiring via synthetic CRISPRa circuits gated by concurrent gain/loss of transcription factors. Nat Commun 2022, 13(1):1454.

3．Xiang C, Zhang YN, Chen QL, Sun AN, Peng YM, Zhang GX, Zhou DX, Xie YY, Hou XS, Zheng FF, Wang F, Gan Z, Chen S*, Liu G*. Increased glycolysis in skeletal muscle coordinates with adipose tissue in systemic metabolic homeostasis. J Cell Mol Med.2021 Jul 6; doi: 10.1111/jcmm.16698. 

4．Zhang GX; Xie YY; Zhou Y; Xiang C; Chen L; Zhang CX; Hou XS; Chen J; Zong H; Liu G* (2017) p53 pathway is involved in cell competition during mouse embryogenesis. Proc Natl Acad Sci U S A. 2017 Jan 17; 114(3):498-503. Epub 2017 Jan 3.

5．Xiao Q, Zhang GX, Wang HJ, Chen L, Lu SS, Pan DJ, Liu G* and Yang ZZ* (2017) A p53 based genetic tracing system to follow postnatal cardiomyocyte expansion in heart regeneration. Development. 2017 Feb 15; 144(4):580-589. doi: 10.1242/dev.147827. Epub 2017 Jan 13.

6．Zhang CX, Zhang Q, Xie YY, He XY, Xiang C, Hou XS, Zhou Y, Chen L, Zhang GX and Liu G*. (2016) MDM 2 actively suppresses p53 activity in oocytes during mouse folliculogenesis. Am J Pathol. 2017 Feb; 187(2):339-351. doi: 10.1016/j.ajpath.2016.09.023. Epub 2016 Nov 29.

7．He XY, Zhang CX, Xiang C, Xie YY, Chen L, Zhang GX and Liu G*.(2015) p53 in myeloid lineage modulates an inflammatory microenvironment limiting initiation and invasion of intestinal tumors. Cell Rep. 2015 Nov 3; 13(5):888-97. doi: 10.1016/j.celrep.2015.09.045.

8．Chen L, Zhang GX, He XY, Zhang CX, Xie YY and Liu G*. (2015) BAC transgenic mice provide evidence that p53 expression is highly regulated in vivo. Cell Death Dis. 2015 Sep 17; 6: e1878. doi: 10.1038/cddis.2015.224.

9．Wang J, Zhu HH, Chu M, Liu Y, Zhang C, Liu G, Yang X, Yang R, Gao WQ. (2014) Symmetrical and asymmetrical division analysis provides evidence for a hierarchy of prostate epithelial cell lineages. Nat Commun. 5:4758. doi: 10.1038/ncomms5758.

10．Zhang Q, He X, Chen L, Zhang C, Gao X, Yang Z, Liu G*. (2012) Synergistic regulation of p53 by Mdm2 and Mdm4 is critical in cardiac endocardial cushion morphogenesis during heart development. J Pathol. 228(3):416-28.

11．Gu X, Xing L, Shi G, Liu Z, Wang X, Qu Z, Wu X, Dong Z, Gao X, Liu G, Yang L and Xu Y. (2011) The circadian mutation PER2(S662G) is linked to cell cycle progression and tumorigenesis. Cell Death Differ. 19(3):397-405.

12．Liu G, Xiong S, Audiffred A, Van Pelt C, Terzian T and Lozano G. (2007) The p53-Mdm2 network in progenitor cell expansion during mouse postnatal development. J Pathol. 213(4):360-8.

13．Barboza JA, Liu G, Ju Z, El-Naggar AK, Lozano G. (2006) p21 delays tumor onset by preservation of chromosomal stability. Proc Natl Acad Sci U S A. 103(52): 19842-19847.

14．Xiong S, Van Pelt C, Elizondo-Fraire AC, Liu G and Lozano G. (2006) Synergistic roles of MdM2 and MdM4 for p53 inhibition in central nervous system development. Proc Natl Acad Sci U S A. 103(9): 3226-3231.

15．Liu G, Lozano G. (2005) p21 Stability: Linking chaperones to a cell cycle checkpoint. Cancer Cell 7(2): 113-114.

16．Liu G, Parant JM, Lang G, Chau P, Chavez-Reyes A, El-Naggar AK, Multani A, Chang S, Lozano G. (2004) Chromosome stability, in the absence of apoptosis, is critical for suppression of tumorigenesis in Trp53 mutant mice. Nature Genetics 36(1): 63-8.(Cover Story).

17．Lang GA, Iwakuma T, Suh Y-A, Liu G, Rao VA, Parant JM, Valentin-Vega YA, Terzian T, Caldwell LC, Strong LC, El-Naggar AL, Lozano G. (2004) Gain-of-Function of a p53 hotspot mutation in a mouse model of Li-Fraumeni syndrome. Cell 119(6): 861-872.

18．Liu G, McDonnell TJ, Montes de Oca Luna R, Kapoor M, Mims B, El-Naggar AK, Lozano G. (2000) High metastatic potential in mice inheriting a targeted p53 missense mutation. Proc Natl Acad Sci U S A. 97(8): 4174-9.