张青实验室

研究方向：

1）Hedgehog（Hh）信号通路调节与人类疾病

       信号转导就是各类信号包括分子信号或外源刺激信号等通过细胞膜或细胞内信使分子介导，最终引起核内靶基因表达改变的过程。

       Hedgehog (Hh)信号通路在正常的胚胎发育中起到关键作用,它的功能障碍可能引发多种人类疾病和肿瘤,其中就包括皮肤癌(基底细胞癌BCC) 、脑癌、肺癌、前列腺癌、胰腺癌和其它消化道器官癌。因此,Hh信号通路的研究对基础科学和临床科学非常重要。

       Hh通路在不同物种间是保守的，本实验室主要以果蝇为模式动物，运用遗传学,生物化学及分子生物学手段研究Hh信号通路是如何调节的。通过研究寻找特异性的调节Hh信号通路的分子靶点，为研发相关的抗肿瘤新药提供理论依据。

2）线粒体功能异常与人类疾病

       线粒体在能量代谢、自由基产生、衰老、细胞凋亡中起重要作用。线粒体相关基因突变，呼吸链缺陷，线粒体膜的改变等因素均会影响整个细胞的正常功能，从而导致病变。许多研究表明，线粒体功能异常与帕金森氏症，阿尔兹海默病，糖尿病，肿瘤等疾病的发生发展过程密切相关。

1.    Zhou Z#, Yao X#, Pang S, Chen P, Jiang W, Shan Z, Zhang Q*. (2017) The deubiquitinase UCHL5/UCH37 positively regulates Hedgehog signaling by deubiquitinating Smoothened. J Mol Cell Biol. 10(3):243-257.

2.    Chen P#, Zhou Z#, Yao X, Pang S, Liu M, Jiang W, Jiang J*, Zhang Q*. (2017) Capping Enzyme mRNA-cap/RNGTT Regulates Hedgehog Pathway Activity by Antagonizing Protein Kinase A. Sci Rep. 7(1): 2891.

3.    Meng H, Cao Y, Qin J, Song X, Zhang Q, Shi Y and Cao L. (2015) DNA methylation, its mediators and genome integrity. Int J Biol Sci. 11(5):604-617.

4.    Zhou Z#, Yao X#, Li S#, Xiong Y, Dong X, Zhao Y, Jiang J*, Zhang Q*. (2015) Deubiquitination of Ci/Gli by Usp7/HAUSP Regulates Hedgehog Signaling. Dev Cell. 34(1):58-72.

5.    Zhou Z, Xu C, Chen P, Liu C, Pang S, Yao X, Zhang Q*. (2015) Stability of HIB-Cul3 E3 ligase adaptor HIB Is Regulated by Self-degradation and Availability of Its Substrates. Sci Rep. 12;5:12709. doi: 10.1038/srep12709.

6.    An J, Ren S, Murphy S, Dalangood S, Chang C, Pang X, Cui Y, Wang L, Pan Y, Zhang X, Zhu Y, Wang C, Halling G, Cheng L, Sukov W, Karnes R, Vasmatzis G, Zhang Q, Zhang J, Cheville J, Yan J, Sun Y, Huang H. (2015) Truncated ERG Oncoproteins from TMPRSS2-ERG Fusions Are Resistant to SPOP-Mediated Proteasome Degradation. Mol Cell. 59:1-13. 

7.    Liu C, Zhou Z, Chen P, Su MY, Chang CJ, Yan J, Jiang J*, Zhang Q*. (2014) Hedgehog signaling downregulates Suppressor of Fused through the HIB/SPOP-Crn axis in Drosophila. Cell Research. 24,595-609.

8.    Zhang Q, Shi Q, Chen Y, Yue T, Li S, Wang B, Jiang J. (2009) Multiple Ser/Thr-rich degrons mediate the degradation of Ci/Gli by the Cul3-HIB/SPOP E3 ubiquitin ligase. PNAS . 106(50):21191-6.

9.    Zhang L, Ren F, Zhang Q, Chen Y, Wang B, Jiang J. (2008) The TEAD/TEF Family of Transcription Factor Scalloped Mediates Hippo Signaling in Organ Size Control. Dev Cell. 14, 377-387. 

10.  Zhang Q#,  Zhang L#, Wang B, Ou CY,  Chien CT, Jiang J. (2006) A Hedgehog-induced BTB protein modulates Hedgehog signaling responses by degrading Ci/Gli transcription factor. Dev Cell. 10, 719-729.

11.  Tao J, Fan C, Gao S, Wang H, Liang G and Zhang Q. (2006) Depression of biofilm formation and antibiotic resistance by sarA disruption in Staphylococcus epidermidis. World J Gastroenterol 12(25): 4009-4013.

12.  Jia J#, Zhang L#, Zhang Q#, Tong C, Wang B, Hou F, Amanai K, Jiang J. (2005) Phosphorylation of Cubitus interruptus by Double-time/CKIe and CKIa targets it   for Slimb/b-TRCP mediated proteolytic processing. Dev Cell. 9, 819-830.

13.  Zhang Q, Zhu MW, Yang YQ, Shao M, Zhang ZY, Lan HY, Yan WY, Wu JJ, Zheng ZX. (2003) A recombinant fusion protein and DNA vaccines against foot-and-mouth disease virus type Asia 1 infection in guinea pigs. Acta Virol. 47(4):237-43.

14.  Zhang Q, Yang YQ, Zhang ZY, Li L, Yan WY, Jiang WJ, Xin AG, Lei CX, Zheng ZX. (2002) Immunogenicity of a recombinant fusion protein of tandem repeat epitopes of foot-and-mouth disease virus type Asia 1 for guinea pigs. Acta Virol 46(1):1-9.